Yang, “Weight loss before a diagnosis of type 2 diabetes mellitus is a risk factor for diabetes complications,” Med. A, Weil, “Biokimia Harper”, Edisi 30, Penerbit Buku Kedokteran EG, Jakarta, 2017. ES, “Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb.) Wedd),” Pharmacy, vol. Lenzen, “The mechanisms of alloxan- and streptozotocin-induced diabetes,” Diabetologia, vol. Noor, “Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province,” Indo. Terhadap Sel β Pankreas Mencit Putih Yang Diinduksi Aloksan Secara Histologis,” J. Tjong, “Efek Ekstrak Etanol Rimpang Temu Mangga Curcuma mangga. K.D, “Aktivitas kemoprevensi ekstrak temu mangga,” Makara, Kesehatan, vol. Gani, “BLOOD GLUCOSE LEVEL AND PANCREAS HISTOLOGICAL SECTION OF MICE ( Mus musculus L.) INDUCED BY ALLOXAN AFTER TREATMENT OF Curcuma mangga Val. Jones, “A Review of Islet of Langerhans Degeneration in Rodent Models of Type 2 Diabetes,” Toxicol. Ninth Edition, International Diabetes Federation, 2019. Greenstein, B., dan Diana, W, “At a Glance Sistem Endokrin”, Edisi Kedua, Erlangga, Jakarta, 2010. C dan Hall, J.E, “Buku Ajar Fisiologi Kedokteran”, Edisi 11, Penerbit Buku Kedokteran EGC, Jakarta, 2007. The activity of lowering blood glucose levels is thought to be influenced by the content of terpenoid compounds. The decrease in blood glucose levels of mice that were given the ethyl acetate fraction of the temu mangga rhizome at doses of 100, 200 and 300 mg/KgBW showed a significant difference compared to the negative control, and the dose of 300 mg/KgBW showed an insignificant difference compared to the positive control. The results showed that the administration of the ethyl acetate fraction of the mangga rhizome at doses of 100, 200 and 300 mg/KgBW prevented weight loss in mice compared to negative controls (p<0.05). The research data were statistically analyzed using two-way ANOVA for the parameters of the percentage decrease in blood glucose and the percentage change in body weight followed by the Tukey multiple range test. The aim of this study was to examine the activity of reducing blood glucose levels in diabetic mice induced by alloxan after administration of the ethyl acetate fraction of temu mangga rhizome at a dose of 100 200 and 300 mg/KgBW were compared with negative control (Na CMC) and positive control (Glibenclamide 0.65 mg/Kg BW). The rhizome of the temu mangga is a member of the Zingiberaceae family that has been used since ancient times for treatment of diabetes.